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human im 9 lymphocytes  (ATCC)


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    ATCC human im 9 lymphocytes
    Human Im 9 Lymphocytes, supplied by ATCC, used in various techniques. Bioz Stars score: 95/100, based on 453 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/human im 9 lymphocytes/product/ATCC
    Average 95 stars, based on 453 article reviews
    human im 9 lymphocytes - by Bioz Stars, 2026-03
    95/100 stars

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    MFRV and LCDV-Sa-VILPs can bind to human IR and IGF1R and stimulate autophoshorylation of the receptors. A-C : Binding competition dose–response curves showing the ability of VILPs to compete with 125-I labeled human insulin for binding to IR-A (A) and IR-B (B) , and with 125-I labeled human IGF-1 for binding to IGF1R (C) . The experiments were performed using <t>IM-9</t> cells for measurements on IR-A binding, while R-/IR-B and R + cells were used for measurements on IR-B and IGF1R, respectively. A representative curve for each peptide to each receptor is shown. Each point represents the mean ± SEM of duplicates, and each experiment was repeated at least three times. D-F: Dose–response curves for ligand-induced autophosphorylation of IR-A and IGF1R. HEK293 cells overexpressing human IR-A (D) or IGF1R (E and F) were used. In F , ligands at indicated concentrations were co-incubated with 5 nM IGF-1. Each point represents the mean ± SEM of duplicates (n ≥ 2).
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    MFRV and LCDV-Sa-VILPs can bind to human IR and IGF1R and stimulate autophoshorylation of the receptors. A-C : Binding competition dose–response curves showing the ability of VILPs to compete with 125-I labeled human insulin for binding to IR-A (A) and IR-B (B) , and with 125-I labeled human IGF-1 for binding to IGF1R (C) . The experiments were performed using <t>IM-9</t> cells for measurements on IR-A binding, while R-/IR-B and R + cells were used for measurements on IR-B and IGF1R, respectively. A representative curve for each peptide to each receptor is shown. Each point represents the mean ± SEM of duplicates, and each experiment was repeated at least three times. D-F: Dose–response curves for ligand-induced autophosphorylation of IR-A and IGF1R. HEK293 cells overexpressing human IR-A (D) or IGF1R (E and F) were used. In F , ligands at indicated concentrations were co-incubated with 5 nM IGF-1. Each point represents the mean ± SEM of duplicates (n ≥ 2).
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    MFRV and LCDV-Sa-VILPs can bind to human IR and IGF1R and stimulate autophoshorylation of the receptors. A-C : Binding competition dose–response curves showing the ability of VILPs to compete with 125-I labeled human insulin for binding to IR-A (A) and IR-B (B) , and with 125-I labeled human IGF-1 for binding to IGF1R (C) . The experiments were performed using IM-9 cells for measurements on IR-A binding, while R-/IR-B and R + cells were used for measurements on IR-B and IGF1R, respectively. A representative curve for each peptide to each receptor is shown. Each point represents the mean ± SEM of duplicates, and each experiment was repeated at least three times. D-F: Dose–response curves for ligand-induced autophosphorylation of IR-A and IGF1R. HEK293 cells overexpressing human IR-A (D) or IGF1R (E and F) were used. In F , ligands at indicated concentrations were co-incubated with 5 nM IGF-1. Each point represents the mean ± SEM of duplicates (n ≥ 2).

    Journal: Molecular Metabolism

    Article Title: A viral insulin-like peptide inhibits IGF-1 receptor phosphorylation and regulates IGF1R gene expression

    doi: 10.1016/j.molmet.2023.101863

    Figure Lengend Snippet: MFRV and LCDV-Sa-VILPs can bind to human IR and IGF1R and stimulate autophoshorylation of the receptors. A-C : Binding competition dose–response curves showing the ability of VILPs to compete with 125-I labeled human insulin for binding to IR-A (A) and IR-B (B) , and with 125-I labeled human IGF-1 for binding to IGF1R (C) . The experiments were performed using IM-9 cells for measurements on IR-A binding, while R-/IR-B and R + cells were used for measurements on IR-B and IGF1R, respectively. A representative curve for each peptide to each receptor is shown. Each point represents the mean ± SEM of duplicates, and each experiment was repeated at least three times. D-F: Dose–response curves for ligand-induced autophosphorylation of IR-A and IGF1R. HEK293 cells overexpressing human IR-A (D) or IGF1R (E and F) were used. In F , ligands at indicated concentrations were co-incubated with 5 nM IGF-1. Each point represents the mean ± SEM of duplicates (n ≥ 2).

    Article Snippet: Human IM-9 lymphocytes (LGC Standards Sp. z.o.o., in partnership with ATCC, #CCL-159, Poland) and murine embryonic fibroblasts, that were derived from IGF1R knockout mice [ ] and stably transfected with either IR-A (R − /IR-A cells), IR-B (R − /IR-B cells) or IGF1R (R + cells) [ ], kindly provided by A. Belfiore (Catanzarro, Italy) and R. Baserga (Philadelphia, PA), were cultured as described previously [ , ].

    Techniques: Binding Assay, Labeling, Incubation